Cystatin F Recombinant Rabbit Monoclonal Antibody [PSH09-00]
cat.: HA723053
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH09-00 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cystatin F aa 1-145. |
| Positive control: | U-937 cell lysate, mouse spleen tissue. |
| Subcellular location: | Secreted, Cytoplasm. |
| Recommended Dilutions:
WB IHC-Fr |
1:2,000 1:100 |
| Uniprot #: | SwissProt: O76096 Human | O89098 Mouse |
| Alternative names: | CMAP CST 7 Cst7 Cystatin 7 cystatin F (leukocystatin) Cystatin like metastasis associated protein Cystatin-7 Cystatin-F Cystatin-like metastasis-associated protein Cystatin7 CystatinF CYTF_HUMAN dJ568C11.1 Leukocystatin |
Images
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Fig1:
Western blot analysis of Cystatin F on different lysates with Rabbit anti-Cystatin F antibody (HA723053) at 1/2,000 dilution. Lane 1: U-937 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (negative) (20 µg/Lane) Predicted band size: 16 kDa Observed band size: 20 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723053) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-Cystatin F antibody (HA723053) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723053, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunofluorescence analysis of frozen mouse brain tissue (negative) with Rabbit anti-Cystatin F antibody (HA723053) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723053, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.