Human Von Willebrand Factor Recombinant Rabbit Monoclonal Antibody [PSH09-04]
cat.: HA723059
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH09-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human von Willebrand factor protein aa 764-1263 (HA210992). |
| Positive control: | Recombinant human von Willebrand factor protein (HA210992). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-03] to Human Von Willebrand Factor antibody (Capture) (HA723058) and Recombinant Human Von Willebrand Factor protein (HA210992) as the standard. The reference range value is 370-30,000 pg/ml. |
| Uniprot #: | SwissProt: P04275 Human |
| Alternative names: | Coagulation factor VIII Coagulation factor VIII VWF F8VWF Factor VIII related antigen von Willebrand antigen 2 von Willebrand antigen II Von Willebrand disease Von Willebrand factor precursor VWD vWF VWF_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723058) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Von Willebrand Factor protein (HA210992) starting from 60000 pg/ml to 0 pg/ml and detect antibody (HA72359, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Von Willebrand Factor in human samples. Interpolated concentration of native Von Willebrand Factor was measured in duplicate at different sample concentrations and interpolated from the Von Willebrand Factor standard curves. Undiluted samples were 2% human serum and 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 1.73 ug/mL in human serum. There was no detectable signal in Hela cell supernatant. |
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Fig3:
Interpolated concentrations of spiked Von Willebrand Factor in cell culture media samples. The concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.