Human CCL23 Recombinant Rabbit Monoclonal Antibody [PSH09-06]
cat.: HA723062
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH09-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL23 aa 22-120. |
| Positive control: | Recombinant Human CCL23 protein (HA210894). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-07] to Human CCL23 antibody (Detector) (HA723063) and Recombinant Human CCL23 protein (HA210894) as the standard. The reference range value is 8-2,000pg/ml. |
| Uniprot #: | SwissProt: P55773 Human |
| Alternative names: | C C motif chemokine ligand 23 C-C motif chemokine 23 C6 beta chemokine CCL 23 CCL23 CCL23(30-99) CCL23_HUMAN Chemokine (C C motif) ligand 23 Chemokine CC motif ligand 23 ck beta 8 CK-beta-8 CKB 8 CKB-8 Ckb-8-1 CKB8 Hmrp-2a Macrophage inflammatory protein 3 MIP 3 MIP-3 MIP3 MPIF 1 MPIF-1 MPIF1 Myeloid progenitor inhibitory factor 1 OTTHUMP00000163948 SCYA 23 SCYA23 Small inducible cytokine A 23 Small inducible cytokine subfamily A (Cys-Cys), member 23 Small inducible cytokine subfamily A, member 23 Small-inducible cytokine A23 |
Images
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Fig1:
Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723062) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human CCL23 (HA210894) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723063, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL23 in human serum samples. The concentrations of CCL23 were measured in duplicates, interpolated from the CCL23 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL23 concentration was determined to be 679.6 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of spiked CCL23 in human cell culture media samples. The concentrations of CCL23 were interpolated from the CCL23 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.