CYR61 / CCN1 Recombinant Rabbit Monoclonal Antibody [PSH09-12]
cat.: HA723070
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH09-12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CCN1 aa 1-381. |
| Positive control: | Saos-2 cell lysate, 786-0 cell lysate, Saos-2, human colon tissue, human skin tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:2,000 1:2,000 1:200 1:50 |
| Uniprot #: | SwissProt: O00622 Human |
| Alternative names: | 3CH61 CCN 1 CCN family member 1 CCN1 CYR 61 Cyr61 CYR61 protein CYR61_HUMAN Cysteine rich angiogenic inducer 61 Cysteine rich heparin binding protein 61 Cysteine-rich angiogenic inducer 61 GIG 1 GIG1 IBP-10 IGF-binding protein 10 Igfbp 10 IGFBP-10 Igfbp10 Insulin like growth factor binding protein 10 Insulin-like growth factor-binding protein 10 Protein CYR61 Protein GIG1 |
Images
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Fig1:
Western blot analysis of CYR61 / CCN1 on different lysates with Rabbit anti-CYR61 / CCN1 antibody (HA723070) at 1/2,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: 786-0 cell lysate Lane 3: MCF7 cell lysate (negative) Lysates/proteins at 15 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 27 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723070) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of untreated Saos-2 (top, positive) / Saos-2 treated with 10μg/mL BFA overnight (middle, positive) / MCF7 (bottom, negative) labeling CYR61 / CCN1 with Rabbit anti-CYR61 / CCN1 antibody (HA723070) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CYR61 / CCN1 antibody (HA723070) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CYR61 / CCN1 antibody (HA723070) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723070) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CYR61 / CCN1 antibody (HA723070) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723070) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Application: IF-Tissue Species: Human Site: colon Sample: Paraffin-embedded section Antibody concentration: 1/50 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.