TDP43 Recombinant Rabbit Monoclonal Antibody [PSH09-14]
cat.: HA723072
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, IHC-Fr, IF-Tissue, FC, IP
Clonality: Monoclonal
Clone number: PSH09-14
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human TDP43 aa 365-414.
Positive control: HeLa cell lysate, K-562 cell lysate, Neuro-2a cell lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate, HeLa, NIH/3T3, human brain tissue, mouse brain tissue, rat brain tissue.
Subcellular location: Cytoplasm, Mitochondrion, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IHC-Fr
  IF-Tissue
  FC
  IP
1:2,000
1:500
1:1,000
1:500
1:200
1:1,000
1-2μg/sample
Uniprot #: SwissProt: Q13148 Human | Q921F2 Mouse
Entrez Gene: 298648 Rat
Alternative names: ALS10 OTTHUMP00000002171 OTTHUMP00000002172 OTTHUMP00000002173 TADBP_HUMAN TAR DNA binding protein 43 TAR DNA binding protein TAR DNA-binding protein 43 TARDBP TDP 43 TDP-43 TDP43
Images
HA723072_1.jpg Fig1: Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723072, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA723072_2.jpg Fig2: Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723072, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA723072_3.jpg Fig3: Western blot analysis of TDP43 on different lysates with Rabbit anti-TDP43 antibody (HA723072) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (40 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: Lane 1-3: 59 seconds; Lane 4-7: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723072) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723072_4.jpg Fig4: Immunocytochemistry analysis of HeLa cells labeling TDP43 with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA723072_5.jpg Fig5: Immunocytochemistry analysis of NIH/3T3 cells labeling TDP43 with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA723072_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TDP43 antibody (HA723072) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723072_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TDP43 antibody (HA723072) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723072_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TDP43 antibody (HA723072) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723072_9.jpg Fig9: Flow cytometric analysis of NIH/3T3 cells labeling TDP43.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA723072, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA723072_10.jpg Fig10: Flow cytometric analysis of C6 cells labeling TDP43.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA723072, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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