Aquaporin 2 Recombinant Rabbit Monoclonal Antibody [PSH09-19]
cat.: HA723077
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH09-19 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Aquaporin 2 aa 215-263/271 |
| Positive control: | Mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Apical cell membrane, Basolateral cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Golgi apparatus, trans-Golgi network membrane |
| Recommended Dilutions:
IHC-P IHC-Fr IF-Tissue |
1:100,000 1:800-1:5,000 1:5,000 |
| Uniprot #: | SwissProt: P41181 Human | P56402 Mouse | P34080 Rat |
| Alternative names: | ADH water channel AQP 2 AQP CD AQP-2 AQP-CD AQP2 AQP2_HUMAN AQPCD Aquaporin 2 collecting duct Aquaporin CD Aquaporin-2 Aquaporin-CD Aquaporin2 Aquaporine 2 Collecting duct water channel protein MGC34501 Water channel aquaporin 2 Water channel protein for renal collecting duct WCH CD WCH-CD WCHCD |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/5,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723077, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunofluorescence analysis of frozen rat kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/800 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723077, green) at 1/800 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/100,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723077) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/100,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723077) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunofluorescence analysis of paraffin-embedded mouse kidney tissue labeling Aquaporin 2 with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723077, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.