DMT1 Recombinant Rabbit Monoclonal Antibody [PSH09-20]
cat.: HA723078
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH09-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DMT1 aa 1-400. |
| Positive control: | HEK-293 cell lysate, HepG2 cell lysate, HeLa cell lysate, human brain tissue, SH-SH5Y. |
| Subcellular location: | Mitochondrion outer membrane, Golgi apparatus, trans-Golgi network membrane, Recycling endosome membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P49281 Human |
| Alternative names: | DCT 1 dct-1 DCT1 Divalent cation transporter 1 Divalent metal transporter 1 DMT 1 DMT-1 DMT1 FLJ37416 Natural resistance associated macrophage protein 2 Natural resistance-associated macrophage protein 2 NRAM2_HUMAN NRAMP 2 NRAMP2 OK/SW-cl.20 Slc11a2 Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 Solute carrier family 11 member 2 |
Images
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Fig1:
Western blot analysis of DMT1 on different lysates with Rabbit anti-DMT1 antibody (HA723078) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (no heat) Lane 2: HepG2 cell lysate (no heat) Lane 3: HeLa cell lysate (no heat) Lane 4: HeLa cell lysate (hot lysis) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723078) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DMT1 antibody (HA723078) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723078) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of SH-SH5Y cells labeling DMT1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723078, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.