Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [PSH09-27]
cat.: HA723085
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: PSH09-27
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 87 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Integrin beta 3 aa 101-150.
Positive control: U-87 MG cell lysate, LNCaP cell lysate, HUVEC cell lysate, C2C12 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, human kidney tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.
Subcellular location: Cell membrane, Cell projection, lamellipodium membrane, Cell junction, focal adhesion, Postsynaptic cell membrane, Synapse.
Recommended Dilutions:
  WB
  IHC-P
1:5,000
1:2,000
Uniprot #: SwissProt: P05106 Human | O54890 Mouse
Entrez Gene: 29302 Rat
Alternative names: BDPLT16 BDPLT2 CD 61 CD61 CD61 antigen GP3A GPIIIa GT HPA 1 HPA 4 Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) Integrin beta chain beta 3 Integrin beta-3 ITB3_HUMAN ITG B3 ITGB 3 ITGB3 NAIT platelet fibrinogen receptor Platelet fibrinogen receptor beta subunit Platelet fibrinogen receptor, beta subunit Platelet glycoprotein IIIa Platelet glycoprotein IIIa precursor Platelet membrane glycoprotein IIIa PTP
Images
HA723085_1.jpg Fig1: Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/5,000 dilution.

Lane 1: U-87 MG cell lysate (20 µg/Lane)
Lane 2: LNCaP cell lysate (low expression) (20 µg/Lane)
Lane 3: HUVEC cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (20 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)

Predicted band size: 87 kDa
Observed band size: 100 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723085) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723085_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723085_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723085_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723085_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.