Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [PSH09-32]
cat.: HA723090
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH09-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CKB aa 1-381. |
| Positive control: | HeLa cell lysate, SH-SY5Y cell lysate, Y79 cell lysate, C6 cell lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, SH-SY5Y. |
| Subcellular location: | Cytoplasm, cytosol, Mitochondrion, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P12277 Human | Q04447 Mouse | P07335 Rat |
| Alternative names: | B CK B-CK BB-CK BCK Brain creatine kinase Ckb CKBB Creatine kinase B Creatine kinase B chain Creatine kinase B type Creatine kinase B-type Creatine Kinase BB Isoenzyme Creatine kinase brain Creatine kinase brain type Creatine phosphokinase BB Epididymis luminal protein 211 Epididymis secretory protein Li 29 HEL 211 HEL S 29 KCRB_HUMAN |
Images
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Fig1:
Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Y79 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse cerebellum tissue lysate Lane 6: Rat cerebellum tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723090) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling Creatine kinase B type with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of SH-SY5Y cells labeling Creatine kinase B type. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723090, 1/200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/200dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.