Human Granzyme A Recombinant Rabbit Monoclonal Antibody [PSH09-36]
cat.: HA723095
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH09-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Granzyme A aa 29-262. |
| Positive control: | Recombinant Human Granzyme A protein (HA210916). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-37] to Human Granzyme A antibody (Detector) (HA723096) and Recombinant Human Granzyme A protein (HA210916) as the standard. The reference range value is 15.6-2,000 pg/ml. |
| Uniprot #: | SwissProt: P12544 Human |
| Alternative names: | CTL tryptase CTLA3 Cytolytic t cell and natural killer cell specific trypsin like serine protease Cytotoxic T lymphocyte associated serine esterase 3 Cytotoxic T lymphocyte proteinase 1 Cytotoxic T-lymphocyte proteinase 1 Fragmentin 1 Fragmentin-1 GRAA_HUMAN Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3 Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease) Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3) Granzyme A Granzyme A precursor Granzyme-1 GZMA H factor Hanukah factor serine protease Hanukkah factor HF HFSP TSP1 |
Images
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Fig1:
Sandwich ELISA analysis of human Granzyme A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723095) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme A protein (HA210916) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723096, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Granzyme A in NK-92 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are NK-92 cell culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 158.2 ng/ml in NK-92 cell culture supernatant. |
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Fig3:
Interpolated concentrations of native Granzyme A in Jurkat and HL-60 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are Jurkat cell culture supernatant 100% and HL-60 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,633.2 pg/ml in Jurkat cell culture supernatant and undetectable in HL-60 cell culture supernatant. |
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Fig4:
Interpolated concentrations of spiked Granzyme A in human cell culture media samples. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.