Human cIAP2 Recombinant Rabbit Monoclonal Antibody [PSH09-40]
cat.: HA723100
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH09-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human cIAP2 aa 246-604. |
| Positive control: | Recombinant Human cIAP2 Protein (HA210619). |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-39] to Human cIAP2 antibody (Capture) (HA723099) and Recombinant Human cIAP2 protein (HA210619) as the standard. The reference range value is 78-20,000pg/ml. |
| Uniprot #: | SwissProt: Q13489 Human |
| Alternative names: | AIP 1 AIP1 API 2 API2 Apoptosis inhibitor 2 Baculoviral IAP repeat containing 3 Baculoviral IAP repeat containing protein 3 Baculoviral IAP repeat-containing protein 3 BIRC 3 BIRC3 BIRC3_HUMAN C IAP2 C-IAP2 Cellular inhibitor of apoptosis 2 cellular inhibitor of apoptosis protein 2 CIAP 2 CIAP2 HAIP 1 HAIP1 HIAP 1 HIAP-1 HIAP1 IAP homolog C IAP-1 Inhibitor of apoptosis protein 1 MALT 2 MALT2 Mammalian IAP homolog C MIHC RING finger protein 49 RNF49 TNFR2 TRAF signaling complex protein 1 TNFR2 TRAF signalling complex protein TNFR2-TRAF-signaling complex protein 1 |
Images
|
Fig1:
Sandwich ELISA analysis of human cIAP2 matched pair antibodies. Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723099) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human cIAP2 Protein (HA210619) starting from 20000 pg/ml to 0 pg/ml and detect antibody (HA723100, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native cIAP2 in Raji, Ramos and SH-SY5Y extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native cIAP2 was measured in duplicate at different sample concentrations and interpolated from the cIAP2 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean cIAP2 concentration was determined to be 1,390 pg/mL in Raji and 561 pg/mL in Ramos cell extract, There was no detectable signal in SH-SY5Y cell extract. |
|
Fig3:
Interpolated concentrations of spiked cIAP2 in cell culture media samples. The concentrations of cIAP2 were measured in duplicates, interpolated from the cIAP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.