eIF3g Recombinant Rabbit Monoclonal Antibody [PSH09-41]
cat.: HA723103
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH09-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human eIF3g aa 1-320. |
| Positive control: | K-562 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, HeLa. |
| Subcellular location: | Cytoplasm, Nucleus, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:25,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: O75821 Human |
| Alternative names: | 44kDa Anti EIF3S4 eIF 3 delta eIF 3 RNA binding subunit eIF-3 RNA-binding subunit eIF-3-delta eIF3 delta eIF3 p42 eIF3 p44 eIF3g EIF3G_HUMAN EIF3S4 EIF3S4 EIF3S4, formerly Eukaryotic translation initiation factor 3 Eukaryotic translation initiation factor 3 RNA binding subunit Eukaryotic translation initiation factor 3 RNA-binding subunit Eukaryotic translation initiation factor 3 subunit 4 Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD Eukaryotic translation initiation factor 3 subunit G Eukaryotic translation initiation factor 3 subunit p42 Eukaryotic translation initiation factor 3, subunit 4, formerly Subunit 4 (delta 44kD) Subunit 4 delta |
Images
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Fig1:
Western blot analysis of eIF3g on different lysates with Rabbit anti-eIF3g antibody (HA723103) at 1/25,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 45 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723103) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling eIF3g with Rabbit anti-eIF3g antibody (HA723103) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF3g antibody (HA723103) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling eIF3g. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723103, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
eIF3g was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with HA723103 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA723103 at 1/5,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y cell lysate (input) Lane 2: HA723103 IP in SH-SY5Y cell lysate Lane 3: Rabbit IgG instead of HA723103 in SH-SY5Y cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 12 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.