eIF3g Recombinant Rabbit Monoclonal Antibody [PSH09-42]
cat.: HA723104
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH09-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human eIF3g aa 1-320. |
| Positive control: | K-562 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus, perinuclear region. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: O75821 Human | Q9Z1D1 Mouse | Q5RK09 Rat |
| Alternative names: | 44kDa Anti EIF3S4 eIF 3 delta eIF 3 RNA binding subunit eIF-3 RNA-binding subunit eIF-3-delta eIF3 delta eIF3 p42 eIF3 p44 eIF3g EIF3G_HUMAN EIF3S4 EIF3S4 EIF3S4, formerly Eukaryotic translation initiation factor 3 Eukaryotic translation initiation factor 3 RNA binding subunit Eukaryotic translation initiation factor 3 RNA-binding subunit Eukaryotic translation initiation factor 3 subunit 4 Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD Eukaryotic translation initiation factor 3 subunit G Eukaryotic translation initiation factor 3 subunit p42 Eukaryotic translation initiation factor 3, subunit 4, formerly Subunit 4 (delta 44kD) Subunit 4 delta |
Images
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Fig1:
Western blot analysis of eIF3g on different lysates with Rabbit anti-eIF3g antibody (HA723104) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: RAW264.7 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723104) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.