Asymmetric Di-Methyl Arginine (ADMA) Recombinant Rabbit Monoclonal Antibody [PSH09-55]
cat.: HA723117
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | ELISA, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH09-55 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Immunogen Asymmetric Dimethylarginine (ADMA) KLH-conjugated |
| Recommended Dilutions:
ELISA WB |
1:2,000 1:2,000 |
| Alternative names: | ADMA Asymmetrical Dimethylarginine Asymmetric Di-Methyl Arginine |
Images
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Fig1:
Western blot analysis of Asymmetric Di-Methyl Arginine (ADMA) on different lysates with Rabbit anti-Asymmetric Di-Methyl Arginine (ADMA) antibody (HA723117) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T treated with 10nM Type I PRMT inhibitor MS023 for 48 hours cell lysate Lysates/proteins at 30 µg/Lane. Exposure time: 1 minute 22 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723117) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Asymmetric Di-Methyl Arginine (ADMA) on different lysates with Rabbit anti-Asymmetric Di-Methyl Arginine (ADMA) antibody (HA723117) at 1/2,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723117) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Indirect ELISA analysis of ADMA. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.