Mouse IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH09-59]
cat.: HA723121
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH09-59 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IL-1 beta aa 118-269 (P10749). |
| Positive control: | Recombinant Mouse IL-1 beta protein (HA210706). |
| Subcellular location: | Secreted |
| Uniprot #: | SwissProt: P10749 Mouse |
| Alternative names: | Catabolin H1 IFN beta inducing factor IL 1 IL 1 beta IL-1 beta IL1 IL1 BETA IL1B IL1B_HUMAN IL1F2 Interleukin 1 beta Interleukin 1 beta precursor interleukin 1, beta Interleukin-1 beta OAF Osteoclast activating factor OTTHUMP00000162031 Preinterleukin 1 beta Preinterleukin beta Pro interleukin 1 beta |
Images
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Fig1: Indirect ELISA analysis of Mouse IL-1 beta was performed by coating wells of a 96-well plate with 50 µL per well of Mouse IL-1 beta-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of Mouse IL-1 beta monoclonal antibody serial diluted starting from a concentration of 1µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.