Human CD80 Recombinant Rabbit Monoclonal Antibody [PSH09-61]
cat.: HA723123
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH09-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD80 aa 35-242 (HA210994). |
| Positive control: | Recombinant Human CD80 protein (HA210994). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-62] to Human CD80 antibody (Detector) (HA723124) or Rabbit monoclonal [PSH09-63] to Human CD80 antibody (Detector) (HA723125) and Recombinant Human CD80 protein (HA210994) as the standard. The reference range value is 15.6-4,000 pg/ml. |
| Uniprot #: | SwissProt: P33681 Human |
| Alternative names: | Activation B7-1 antigen B lymphocyte activation antigen B7 B7 B7-1 B7-1 antigen B7.1 BB1 CD28 antigen ligand 1 CD28LG CD28LG1 CD80 CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) CD80 antigen CD80 molecule CD80_HUMAN Costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 LAB7 T-lymphocyte activation antigen CD80 |
Images
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Fig1:
Sandwich ELISA analysis of human CD80 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723123) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD80 protein (HA210994) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723124, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of human CD80 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723123) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD80 protein (HA210994) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723125, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of native CD80 in Raji and HUVEC extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD80 was measured in duplicate at different sample concentrations and interpolated from the CD80 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 30,943 pg/mL in Raji cell extract, There was no detectable signal in HUVEC cell extract. |
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Fig4:
Interpolated concentrations of native CD80 in Raji and HUVEC extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD80 was measured in duplicate at different sample concentrations and interpolated from the CD80 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 40,639 pg/mL in Raji cell extract, There was no detectable signal in HUVEC cell extract. |
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Fig5:
Interpolated concentrations of spiked CD80 in cell culture media samples. The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig6:
Interpolated concentrations of spiked CD80 in cell culture media samples. The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.