SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [PSH09-67]
cat.: HA723132
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH09-67
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SLC31A1 / CTR1 aa 80-190/190
Positive control: HepG2 cell lysate, HeLa cell lysate, Caco-2 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate.
Subcellular location: Cell membrane ; Early endosome membrane ; Recycling endosome membrane ; Apical cell membrane ; Late endosome membrane ; Basolateral cell membrane.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: O15431 Human | Q8K211 Mouse | Q9JK41 Rat
Alternative names: Copper transport 1 homolog Copper transporter 1 COPT1 COPT1_HUMAN CTR1 hCTR1 High affinity copper uptake protein 1 SLC31A1 solute carrier family 31 (copper tansporters) member 1 Solute carrier family 31 member 1
Images
HA723132_1.jpg Fig1: Western blot analysis of SLC31A1 / CTR1 on different lysates with Rabbit anti-SLC31A1 / CTR1 antibody (HA723132) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Caco-2 cell lysate (20 µg/Lane)
Lane 4: Neuro-2a cell lysate (20 µg/Lane)
Lane 5: C2C12 cell lysate (20 µg/Lane)
Lane 6: C6 cell lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (20 µg/Lane)

Predicted band size: 21 kDa
Observed band size: 30 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723132) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.