SOCS1 Recombinant Rabbit Monoclonal Antibody [PSH09-68]
cat.: HA723133
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH09-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SOCS1 aa 1-211. |
| Positive control: | K-562 cell lysate, HDLM-2 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, A20 cell lysate, Rat thymus tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O15524 Human | O35716 Mouse | Q9QX78 Rat |
| Alternative names: | CIS1 CISH 1 CISH1 Cytokine inducible SH2 protein 1 JAB JAK binding protein JAK-binding protein Janus kinase binding protein SOCS 1 SOCS-1 Socs1 SOCS1_HUMAN SSI 1 SSI-1 SSI1 STAT induced STAT inhibitor 1 STAT-induced STAT inhibitor 1 Suppressor of cytokine signaling 1 Supressor of cytokine signalling 1 TEC interacting protein 3 Tec-interacting protein 3 TIP 3 TIP-3 TIP3 |
Images
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Fig1:
Western blot analysis of SOCS1 on different lysates with Rabbit anti-SOCS1 antibody (HA723133) at 1/1,000 dilution. Lane 1: K-562 cell lysate (30 µg/Lane) Lane 2: HDLM-2 cell lysate (30 µg/Lane) Lane 3: 293T cell lysate (negative) (30 µg/Lane) Lane 4: COS-1 cell lysate (30 µg/Lane) Lane 5: NIH/3T3 cell lysate (30 µg/Lane) Lane 6: A20 cell lysate (low expression) (30 µg/Lane) Lane 7: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723133) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.