Phospho-STING (S366) Recombinant Rabbit Monoclonal Antibody [PSH09-72]
cat.: HA723137
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, Dot Blot, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH09-72 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser366 of Human STING. |
| Positive control: | THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane, Cytoplasm, perinuclear region, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Mitochondrion outer membrane, Cell membrane. |
| Recommended Dilutions:
WB Dot Blot IP |
1:2,000 1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q86WV6 Human | Q3TBT3 Mouse | F1M391 Rat |
| Alternative names: | endoplasmic reticulum IFN stimulator Endoplasmic reticulum interferon stimulator ERIS FLJ38577 hMITA hSTING Mediator of IRF3 activation MITA Mitochondrial mediator of IRF3 activation MPYS N terminal methionine proline tyrosine serine plasma membrane tetraspanner NET23 Stimulator of interferon genes Stimulator of interferon genes protein STING TM173_HUMAN Tmem173 Transmembrane protein 173 |
Images
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Fig1:
Western blot analysis of Phospho-STING (S366) on different lysates with Rabbit anti-Phospho-STING (S366) antibody (HA723137) at 1/2,000 dilution. Lane 1: THP-1 treated with 80nM TPA for 16 hours cell lysate Lane 2: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate Lane 3: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723137) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Dot blot analysis of Phospho-STING (S366) on different peptides with Rabbit anti-Phospho-STING (S366) antibody (HA723137) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified STING peptide (negative) Lane 2: Phospho-STING (S366) peptide (positive) Proteins loading: 100ng, 50ng, 10ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute; ECL: K1801. |
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Fig3:
Phospho-STING (S366) was immunoprecipitated from 0.2 mg THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate with HA723137 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723137 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate (input) Lane 2: HA723137 IP in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate Lane 3: Rabbit IgG instead of HA723137 in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.