Biotin Conjugated Human TREM2 Recombinant Rabbit Monoclonal Antibody [PSH09-85]
cat.: HA723152B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH09-85 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TREM2 Protein aa 19-174. |
| Positive control: | Recombinant Human TREM2 Protein (HA210647). |
| Subcellular location: | Secreted, Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-83] to Human TREM2 antibody (Capture) (HA723149) and Recombinant Human TREM2 protein (HA210647) as the standard. The reference range value is 39-5,000pg/ml. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9NZC2 Human |
| Alternative names: | TREM 2 TREM-2 TREM2 TREM2_HUMAN TREM2a TREM2b TREM2c Trggering receptor expressed on myeloid cells 2 Trggering receptor expressed on myeloid cells 2a Triggering receptor expressed on monocytes 2 Triggering receptor expressed on myeloid cells 2 |
Images
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Fig1:
Sandwich ELISA analysis of Human TREM2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723149) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TREM2 protein (HA210647) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723152B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native TREM2 in human cell culture supernatant samples. Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations and interpolated from the TREM2 standard curves. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 4,912 pg/mL in THP-1 cell culture supernatant, undetectable in Jurkat cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked TREM2 in cell culture media samples. The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.