Human sTNF RI Recombinant Rabbit Monoclonal Antibody [PSH09-89]
cat.: HA723158
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH09-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TNF RI aa 30-211. |
| Positive control: | Recombinant Human sTNF RI Protein (HA210562). |
| Subcellular location: | Secreted. Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-90] to Human sTNF RI antibody (Detector) (HA723159) and Recombinant Human sTNF RI protein (HA210562) as the standard. The reference range value is 15.6-4,000 pg/ml. |
| Uniprot #: | SwissProt: P19438 Human |
| Alternative names: | CD120a FPF MGC19588 p55 p55-R p60 TBP1 TBPI TNF R TNF R55 TNF-R1 TNF-RI TNFAR TNFR-I TNFR1 TNFR55 TNFR60 TNFRI TNFRSF1a TNR1A_HUMAN Tumor necrosis factor receptor 1 Tumor necrosis factor receptor superfamily, member 1A Tumor necrosis factor receptor type 1 Tumor necrosis factor receptor type I Tumor necrosis factor-binding protein 1 |
Images
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Fig1:
Sandwich ELISA analysis of Human sTNF RI matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723154) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human sTNF RI Protein (HA210562) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723159, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native sTNF R1 in human samples. Interpolated concentration of native sTNF R1 was measured in duplicate at different sample concentrations and interpolated from the sTNF R1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 3,727 pg/mL in human urine and 1,145 pg/ml in A549 cell supernatant. There was no detectable signal in Raji supernatant. |
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Fig3:
Interpolated concentrations of spiked sTNF RI in cell culture media samples. The concentrations of sTNF RI were measured in duplicates, interpolated from the sTNF RI standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.