Phospho-RICTOR (T1135) Recombinant Rabbit Monoclonal Antibody [PSH09-92]
cat.: HA723161
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH09-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 192 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr1135 of Human RICTOR. |
| Positive control: | HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate. |
| Subcellular location: | Cytosol, Golgi apparatus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q6R327 Human | Q6QI06 Mouse |
| Alternative names: | AVO3 AVO3 homolog DKFZp686B11164 hAVO3 KIAA1999 Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) mAVO3 MGC39830 PIA Pianissimo Rapamycin insensitive companion of mTOR Rapamycin-insensitive companion of mTOR Rictor RICTR RICTR_HUMAN RPTOR independent companion of MTOR complex 2 TORC2 specific protein AVO3 |
Images
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Fig1:
Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA723161) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate Lane 3: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 192 kDa Observed band size: 240 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723161) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA723161) at 1/2,000 dilution. Lane 1: C2C12 starved overnight cell lysate Lane 2: C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 192 kDa Observed band size: 240 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723161) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.