CFTR Recombinant Rabbit Monoclonal Antibody [PSH09-99]
cat.: HA723168
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH09-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 168 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CFTR aa 1,181-1,440. |
| Positive control: | Caco-2 cell lysate, HT-29 cell lysate, human pancreas tissue. |
| Subcellular location: | Apical cell membrane, Early endosome membrane, Cell membrane, Recycling endosome membrane, Endoplasmic reticulum membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IP |
1:2,000 1:200 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: P13569 Human |
| Alternative names: | ABC 35 ABC35 ABCC 7 ABCC7 ATP binding cassette sub family C member 7 ATP Binding Cassette Superfamily C Member 7 ATP binding cassette transporter sub family C member 7 ATP-binding cassette sub-family C member 7 cAMP dependent chloride channel cAMP-dependent chloride channel CF CFTR CFTR/MRP CFTR_HUMAN Channel conductance controlling ATPase Channel conductance-controlling ATPase Cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub family C, member 7) Cystic fibrosis transmembrane conductance regulator Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 Cystic Fibrosis Transmembrane Regulator dJ760C5.1 MRP 7 MRP7 TNR CFTR |
Images
|
Fig1:
Western blot analysis of CFTR on different lysates with Rabbit anti-CFTR antibody (HA723168) at 1/2,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HT-29 cell lysate Lane 3: U-2 OS cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 168 kDa Observed band size: 250 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723168) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-CFTR antibody (HA723168) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723168) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Application: IF-Tissue Species: Human Site: pancreas Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.