LPCAT3 Recombinant Rabbit Monoclonal Antibody [PSH10-02]
cat.: HA723171
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Green monkey |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH10-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LPCAT3 aa 1-363. |
| Positive control: | HT-29 cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, HeLa. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: Q6P1A2 Human |
| Alternative names: | 1-acylglycerophosphocholine O-acyltransferase 1-acylglycerophosphoserine O-acyltransferase LPCAT Lpcat3 LPLAT 5 LPSAT Lyso-PC acyltransferase 3 Lyso-PC acyltransferase Lyso-PS acyltransferase Lysophosphatidylcholine acyltransferase 3 Lysophosphatidylcholine acyltransferase Lysophosphatidylserine acyltransferase Lysophospholipid acyltransferase 5 MBOA5 MBOA5_HUMAN Membrane-bound O-acyltransferase domain-containing protein 5 O-acyltransferase domain-containing protein 5 |
Images
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Fig1:
Western blot analysis of LPCAT3 on different lysates with Rabbit anti-LPCAT3 antibody (HA723171) at 1/2,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723171) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling LPCAT3 with Rabbit anti-LPCAT3 antibody (HA723171) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LPCAT3 antibody (HA723171) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.