Glucose Transporter GLUT4 Recombinant Rabbit Monoclonal Antibody [PSH10-06]
cat.: HA723175
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH10-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GLUT4 aa 447-509. |
| Positive control: | Mouse heart tissue, rat skeletal muscle tissue, Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate. |
| Subcellular location: | Cell membrane, Endomembrane system, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IHC-Fr |
1:5,000 1:500 |
| Uniprot #: | SwissProt: P14142 Mouse | P19357 Rat |
| Alternative names: | insulin-responsive Glucose transporter GLUT 4 Glucose transporter type 4 Glucose transporter type 4 insulin responsive GLUT 4 GLUT-4 GLUT4 GTR4_HUMAN Insulin responsive glucose transporter type 4 kug SLC 2A4 SLC2A4 solute carrier family 2 (facilitated glucose transporter) member 4 Solute carrier family 2 member 4 Solute carrier family 2, facilitated glucose transporter member 4 |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse heart tissue with Rabbit anti-Glucose Transporter GLUT4 antibody (HA723175) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723175, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig2:
Immunofluorescence analysis of frozen rat skeletal muscle tissue with Rabbit anti-Glucose Transporter GLUT4 antibody (HA723175) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723175, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Western blot analysis of Glucose Transporter GLUT4 on different lysates with Rabbit anti-Glucose Transporter GLUT4 antibody (HA723175) at 1/5,000 dilution. Lane 1: Mouse heart tissue lysate (no heat) (30 µg/Lane) Lane 2: Mouse skeletal muscle tissue lysate (no heat) (30 µg/Lane) Lane 3: Mouse kidney tissue lysate (no heat) (negative) (30 µg/Lane) Lane 4: Rat kidney tissue lysate (negative) (30 µg/Lane) Lane 5: Rat lung tissue lysate (no heat) (negative) (30 µg/Lane) Lane 6: Rat skeletal muscle tissue lysate (no heat) (30 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723175) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.