GDF15 Recombinant Rabbit Monoclonal Antibody [PSH10-09]
cat.: HA723178
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GDF15 aa 159-308. |
| Positive control: | LNCaP cell lysate, human placenta tissue, human prostate cancer tissue, human breast cancer tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q99988 Human |
| Alternative names: | GDF 15 GDF-15 Gdf15 GDF15_HUMAN Growth differentiation factor 15 Growth/differentiation factor 15 Macrophage inhibitory cytokine 1 MIC 1 Mic-1 MIC1 NAG 1 NAG-1 NAG1 NRG 1 NRG-1 NRG1 NSAID (nonsteroidal anti inflammatory drug) activated protein 1 NSAID NSAID regulated protein 1 NSAID-activated gene 1 protein NSAID-regulated gene 1 protein PDF PLAB Placental bone morphogenetic protein Placental bone morphogenic protein Placental TGF beta Placental TGF-beta Prostate differentiation factor PTGF beta PTGFB |
Images
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Fig1:
Western blot analysis of GDF15 on different lysates with Rabbit anti-GDF15 antibody (HA723178) at 1/2,000 dilution. Lane 1: LNCaP cell lysate Lane 2: K-562 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 34 kDa Observed band size: 35/15 kDa Exposure time: 1 minute 33 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723178) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GDF15 antibody (HA723178) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723178) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-GDF15 antibody (HA723178) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723178) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-GDF15 antibody (HA723178) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723178) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.