TCF7 Recombinant Rabbit Monoclonal Antibody [PSH10-10]
cat.: HA723179
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH10-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TCF7 aa 1-300. |
| Positive control: | MOLT-4 cell lysate, COLO205 cell lysate, Jurkat cell lysate, human thymus tissue, human tonsils tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P36402 Human |
| Alternative names: | FLJ36364 MGC47735 OTTHUMP00000159391 T cell factor 1 T cell specific transcription factor 1 T-cell factor 1 T-cell-specific transcription factor 1 TCF-1 TCF-7 TCF1 Tcf7 TCF7 transcription factor 7 TCF7_HUMAN Transcription factor 7 (T cell specific HMG box) Transcription factor 7 Transcription factor-7, T-cell specific Transcription factor-7, T-cell specific, 1 |
Images
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Fig1:
Western blot analysis of TCF7 on different lysates with Rabbit anti-TCF7 antibody (HA723179) at 1/2,000 dilution. Lane 1: MOLT-4 cell lysate Lane 2: COLO205 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 45-50 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723179) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-TCF7 antibody (HA723179) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723179) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsils tissue with Rabbit anti-TCF7 antibody (HA723179) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723179) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
TCF7 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA723179 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723179 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA723179 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA723179 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.