Human OX40/TNFRSF4 Recombinant Rabbit Monoclonal Antibody [PSH10-18]
cat.: HA723188
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH10-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human OX40/TNFRSF4 aa 29-214 (HA210892). |
| Positive control: | Recombinant Human OX40/TNFRSF4 protein (HA210892). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-17] to Human OX40/TNFRSF4 antibody (Capture) (HA723187) and Recombinant Human OX40/TNFRSF4 protein (HA210892) as the standard. The reference range value is 31.25-8,000 pg/ml. |
| Uniprot #: | SwissProt: P43489 Human |
| Alternative names: | ACT 35 ACT35 ACT35 antigen ATC35 antigen CD 134 CD134 CD134 antigen IMD16 Lymphoid activation antigene ACT35 OX 40 OX40 OX40 antigen OX40 cell surface antigen OX40 homologue OX40L receptor TAX transcriptionally activated glycoprotein 1 receptor TAX transcriptionally-activated glycoprotein 1 receptor TNF receptor superfamily member 4 TNFRSF 4 TNFRSF4 TNR4_HUMAN Tumor necrosis factor receptor superfamily member 4 Txgp 1l Txgp1 Txgp1l |
Images
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Fig1:
Sandwich ELISA analysis of Human OX40/TNFRSF4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723187) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human OX40/TNFRSF4 protein (HA210892) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723188, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native OX40 in human PBMC cell culture supernatant. PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 5 days. The concentrations of OX40 measured, interpolated from the OX40 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The mean OX40 concentration was determined to be 418.6 pg/ml in PHA-M stimulated PBMC cell culture supernatant and undetectable in the unstimulated PBMC control. |
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Fig3:
Interpolated concentrations of spiked OX40 in human cell culture media samples. The concentrations of OX40 were interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 50%, cell culture media 2 50%. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.