CD81 Recombinant Rabbit Monoclonal Antibody [PSH10-26]
cat.: HA723193
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH10-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD81 aa 34-201 |
| Positive control: | Raji cell lysate, HL-60 cell lysate, U-937 cell lysate, PC-12 cell lysate, HCT 116 cell lysate, human liver tissue, Jurkat. |
| Subcellular location: | Cell membrane, Basolateral cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P60033 Human | Q62745 Rat |
| Alternative names: | 26 kDa cell surface protein TAPA 1 26 kDa cell surface protein TAPA-1 26 kDa cell surface protein TAPA1 CD 81 CD81 CD81 antigen (target of antiproliferative 1) CD81 antigen CD81 molecule CD81_HUMAN CVID6 S5.7 TAPA 1 TAPA1 Target of the antiproliferative 1 Tetraspanin 28 Tetraspanin-28 Tetraspanin28 Tspan 28 Tspan-28 Tspan28 |
Images
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Fig1:
Western blot analysis of CD81 on different lysates with Rabbit anti-CD81 antibody (HA723193) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: HL-60 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: PC-12 cell lysate Lane 5: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 20 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723193) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD81 antibody (HA723193) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723193) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of HepG2 (left, negative) and Jurkat (right, positive) cells labeling CD81. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723193, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.