Tau Recombinant Rabbit Monoclonal Antibody [PSH10-42]
cat.: HA723209
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: ELISA(Det)
Clonality: Monoclonal
Clone number: PSH10-42
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Synthetic peptide within human Tau aa 57-68.
Subcellular location: c
Recommended Dilutions:
  ELISA(Det)
  ELISA(Det)
  ELISA(Det)
  ELISA(Det)
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-41] to Human Phospho-Tau (T217) antibody (Capture) (HA723841) and Recombinant Human Phospho-Tau (T217) protein (HA211033) as the standard. The reference range value is 0.195-50 ng/mL.
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-43] to Human Non-Phoshpho-Tau (T217) antibody (Capture) (HA723843) and Recombinant Human Phospho-Tau (T217) protein (HA210937) as the standard. The reference range value is 0.117-30 pg/mL.
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-46] to Brain-derived tau/BD-tau antibody (Capture) (HA723847) and Recombinant Human Tau/Tau441 protein (HA210937) as the standard. The reference range value is 39-10,000 pg/mL.
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-48] to Tau antibody (Capture) (HA723850) and Recombinant Human Tau/Tau441 protein (HA210937) as the standard. The reference range value is 39-5,000 pg/mL.
Uniprot #: SwissProt: P10636-8 Human | P10637 Mouse
Alternative names: AI413597 AW045860 DDPAC FLJ31424 FTDP 17 G protein beta1/gamma2 subunit interacting factor 1 MAPT MAPTL MGC134287 MGC138549 MGC156663 Microtubule associated protein tau Microtubule associated protein tau isoform 4 Microtubule-associated protein tau MSTD Mtapt MTBT1 MTBT2 Neurofibrillary tangle protein Paired helical filament tau Paired helical filament-tau PHF tau PHF-tau PPND PPP1R103 Protein phosphatase 1, regulatory subunit 103 pTau RNPTAU TAU TAU_HUMAN Tauopathy and respiratory failure, included
Images
HA723209_1.png Fig1: Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies

Capture: HA723841, Phospho-Tau (T217) Rabbit mAb [PSH16-41]
Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723841) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723209_2.png Fig2: Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies

Capture: HA723843, Phospho-Tau (T217) Rabbit mAb [PSH16-43]
Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723843) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723209_3.png Fig3: Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies

Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46]
Detector: HA723209, Brain-derived tau/BD-tau Rabbit mAb [PSH10-42]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723209_4.png Fig4: Sandwich ELISA analysis of human Tau matched pair antibodies

Capture: HA723850, Tau Rabbit mAb [PSH16-48]
Detector: HA723209, Tau Rabbit mAb [PSH10-42]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723209_5.png Fig5: Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies

Capture: HA723877, Phospho-Tau (T217) Rabbit mAb [PSH16-75]
Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723877) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 15000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.