VAChT / SLC18A3 Recombinant Rabbit Monoclonal Antibody [PSH10-50]
cat.: HA723214
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N terminal Mouse VAChT. |
| Positive control: | Mouse brain tissue, mouse striatum tissue, rat brain tissue, rat striatum tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:1,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: O35304 Mouse | Q62666 Rat |
| Alternative names: | AChR MGC12716 rVAT Slc18a3 Solute carrier family 18 (vesicular acetylcholine) member 3 Solute carrier family 18 (vesicular monoamine) member 3 Solute carrier family 18 member 3 VAChT VACHT_HUMAN Vesicular acetylcholine transporter |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Western blot analysis of VAChT / SLC18A3 on different lysates with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate (low expression) Lane 3: Rat brain tissue lysate Lane 4: Rat kidney tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723214) at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.