Phospho-eIF4EBP1 (T70) Recombinant Rabbit Monoclonal Antibody [PSH09-91]
cat.: HA723222
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH09-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr70 of human eIF4EBP1. |
| Positive control: | HEK-293 cell lysate, HEK-293 treated with 20% FBS for 3 hours cell lysate, mouse liver tissue, mouse pancreas tissue. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q13541 Human | Q60876 Mouse |
| Alternative names: | 4E-BP1 4EBP1 4EBP1_HUMAN BP 1 eIF4E binding protein 1 eIF4E-binding protein 1 Eif4ebp1 Eukaryotic translation initiation factor 4E-binding protein 1 PHAS-I PHASI Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
Images
|
Fig1:
Western blot analysis of Phospho-eIF4EBP1 (T70) on different lysates with Rabbit anti-Phospho-eIF4EBP1 (T70) antibody (HA723222) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 20% FBS for 3 hours cell lysate Lane 3: HEK-293 treated with 20% FBS for 3 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 13 kDa Observed band size: 17/18 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723222) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Phospho-eIF4EBP1 (T70) antibody (HA723222) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723222) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Phospho-eIF4EBP1 (T70) antibody (HA723222) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723222) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Phospho-eIF4EBP1 (T70) was immunoprecipitated from 0.2 mg HEK-293 cell lysate with HA723222 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723222 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEK-293 cell lysate (input) Lane 2: HA723222 IP in HEK-293 cell lysate Lane 3: Rabbit IgG instead of HA723222 in HEK-293 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.