IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60]
cat.: HA723225
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH10-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IRE1 aa 1-400. |
| Positive control: | HEK-293 cell lysate, Neuro-2a cell lysate, C6 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane; nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O75460 Human | Q9EQY0 Mouse Entrez Gene: 498013 Rat |
| Alternative names: | Endoplasmic reticulum (ER) to nucleus signalling 1 Endoplasmic reticulum to nucleus signaling 1 Endoplasmic reticulum-to-nucleus signaling 1 Endoribonuclease ER to nucleus signaling 1 ERN 1 Ern1 ERN1_HUMAN hIRE 1p hIRE1p Inositol requiring 1 Inositol requiring 1, S. cerevisiae, homolog of Inositol requiring enzyme 1, S. cerevisiae, homolog of Inositol requiring protein 1 inositol-requiring enzyme 1 Inositol-requiring protein 1 IRE 1 IRE 1a IRE 1P Ire1 alpha Ire1-alpha IRE1a Ire1alpha IRE1P MGC163277 MGC163279 Protein kinase/endoribonuclease RGD1559716 Serine/threonine protein kinase/endoribonuclease IRE1 |
Images
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Fig1:
Western blot analysis of IRE1 on different lysates with Rabbit anti-IRE1 antibody (HA723225) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: C6 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723225) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.