SHANK2 Recombinant Rabbit Monoclonal Antibody [PSH10-61]
cat.: HA723226
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH10-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 201 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SHANK2 aa 901-1,230. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, mouse hippocampus tissue, mouse cerebellum tissue, mouse liver tissue. |
| Subcellular location: | Apical cell membrane, Cytoplasm, Synapse, Postsynaptic density, Cell projection, growth cone, Cell projection, dendritic spine. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000 1:50-1:100 1:200 |
| Uniprot #: | SwissProt: Q9UPX8 Human | Q80Z38 Mouse | Q9QX74 Rat |
| Alternative names: | AUTS17 Cortactin binding protein 1 Cortactin SH3 domain-binding protein Cortactin-binding protein 1 CortBP1 CTTNBP1 GKAP/SAPAP interacting protein GKAP/SAPAP-interacting protein KIAA1022 Proline rich synapse associated protein 1 Proline-rich synapse-associated protein 1 ProSAP1 SH3 and multiple ankyrin repeat domains 2 SH3 and multiple ankyrin repeat domains protein 2 SHAN2_RAT SHANK Shank2 SPANK-3 |
Images
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Fig1:
Western blot analysis of SHANK2 on different lysates with Rabbit anti-SHANK2 antibody (HA723226) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (40 µg/Lane) Lane 2: Mouse lung tissue lysate (negative) (40 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Lane 4: Rat lung tissue lysate (negative) (40 µg/Lane) Predicted band size: 201 kDa Observed band size: 159 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723226) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SHANK2 antibody (HA723226) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723226) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-SHANK2 antibody (HA723226) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723226) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SHANK2 antibody (HA723226) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723226) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SHANK2 antibody (HA723226) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723226) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling SHANK2 with Rabbit anti-SHANK2 antibody (HA723226) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723226, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.