VPS34 Recombinant Rabbit Monoclonal Antibody [PSH10-64]
cat.: HA723229
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH10-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human VPS34 aa 251-887. |
| Positive control: | HeLa cell lysate, PC-3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Mouse brain tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Midbody, Late endosome, Cytoplasmic vesicle, autophagosome. |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: Q8NEB9 Human | Q6PF93 Mouse | O88763 Rat |
| Alternative names: | hVps34 MGC61518 Phosphatidylinositol 3 kinase catalytic subunit type 3 Phosphatidylinositol 3 kinase class 3 Phosphatidylinositol 3 kinase p100 subunit Phosphatidylinositol 3-kinase catalytic subunit type 3 Phosphatidylinositol 3-kinase p100 subunit Phosphoinositide 3 kinase class 3 Phosphoinositide-3-kinase class 3 PI3 kinase type 3 PI3-kinase type 3 PI3K type 3 Pik3c3 PK3C3_HUMAN PtdIns 3 kinase type 3 PtdIns-3-kinase type 3 Vps 34 Vps34 |
Images
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Fig1:
Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (HA723229) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: PC-3 cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 102 kDa Observed band size: 98 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723229) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (HA723229) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-VPS34 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 98 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723229) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.