CD48 Recombinant Rabbit Monoclonal Antibody [PSH10-65]
cat.: HA723230
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD48 aa 27-220. |
| Positive control: | Raji cell lysate, Ramos cell lysate, Daudi cell lysate, Jurkat cell lysate, Daudi, human colon tissue, human tonsil tissue, human lung adenocarcinoma tissue. |
| Subcellular location: | Cell membrane, Membrane raft, Secreted. |
| Recommended Dilutions:
WB FC IP IHC-P |
1:2,000 1:2,000 1-2μg/sample 1:600-1:2,000 |
| Uniprot #: | SwissProt: P09326 Human |
| Alternative names: | Antigen CD48 B cell membrane protein B lymphocyte activation marker BLAST 1 B-cell activation marker B-lymphocyte activation marker BLAST-1 BCM 1 surface antigen BCM1 BCM1 surface antigen BLAST 1 BLAST BLAST1 CD 48 CD48 CD48 antigen (B cell membrane protein) CD48 antigen CD48 molecule CD48 protein CD48_HUMAN hCD48 Leucocyte antigen MEM 102 Leukocyte antigen MEM-102 mCD48 MEM 102 MEM-102 MEM102 Signaling lymphocytic activation molecule 2 SLAM family member 2 SLAMF 2 SLAMF2 TCT.1 |
Images
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Fig1:
Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA723230) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: Ramos cell lysate Lane 4: THP-1 cell lysate (negative) Lane 5: Daudi cell lysate Lane 6: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 40-50 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723230) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HeLa (left, negative) and Daudi (right, positive) cells labeling CD48. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723230, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Flow cytometric analysis of THP-1 (left, negative) and Daudi (right, positive) cells labeling CD48. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723230, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
CD48 was immunoprecipitated from 0.2 mg Daudi cell lysate with HA723230 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723230 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Daudi cell lysate (input) Lane 2: HA723230 IP in Daudi cell lysate Lane 3: Rabbit IgG instead of HA723230 in Daudi cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 seconds; ECL: K1801 |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD48 antibody (HA723230) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723230) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD48 antibody (HA723230) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723230) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-CD48 antibody (HA723230) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723230) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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