Arc / Arg3.1 Recombinant Rabbit Monoclonal Antibody [PSH10-67]
cat.: HA723231
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH10-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Arc aa 1-396. |
| Positive control: | Mouse brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Extracellular vesicle membrane. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:2,000 1:200 1:500 |
| Uniprot #: | SwissProt: Q9WV31 Mouse | Q63053 Rat |
| Alternative names: | activity regulated cytoskeleton associated protein activity regulated gene 3.1 protein homolog Activity-regulated cytoskeleton-associated protein Activity-regulated gene 3.1 protein homolog Arc ARC/ARG3.1 ARC_HUMAN Arg 3.1 Arg3.1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Arc / Arg3.1 antibody (HA723231) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723231) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Arc / Arg3.1 antibody (HA723231) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723231) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Western blot analysis of Arc / Arg3.1 on different lysates with Rabbit anti-Arc / Arg3.1 antibody (HA723231) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate (low expression) Lane 3: Rat brain tissue lysate Lane 4: Rat kidney tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723231) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.