Human IL-18BP Recombinant Rabbit Monoclonal Antibody [PSH10-71]
cat.: HA723235
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH10-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-18BP aa 31-194 (HA210686). |
| Positive control: | Recombinant Human IL-18BP protein (HA210686). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-72] to Human IL-18BP antibody (Detector) (HA723236) and Recombinant Human IL-18BP protein (HA210686) as the standard. The reference range value is 39-5,000 pg/ml. |
| Uniprot #: | SwissProt: O95998 Human |
| Alternative names: | I18BP_HUMAN IL-18BP IL18 binding protein IL18 BP IL18 BPa IL18BP IL18BPa Interleukin 18 binding protein Interleukin-18-binding protein MC51L 53L 54L homolog gene product Tadekinig alfa Tadekinig-alfa |
Images
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Fig1:
Sandwich ELISA analysis of human IL-18BP matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723235) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-18BP protein (HA210686) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723236, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL-18BP in human urine samples. The concentrations of IL-18BP were measured in duplicates, interpolated from the IL-18BP standard curve and corrected for sample dilution. Undiluted samples are human urine 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18BP concentration was determined to be 56,552 pg/ml in human urine. |
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Fig3:
Interpolated concentrations of spiked IL-18BP in human cell culture media samples. The concentrations of IL-18BP were measured in duplicates, interpolated from the IL-18BP standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.