Human MICB Recombinant Rabbit Monoclonal Antibody [PSH10-74]
cat.: HA723239
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH10-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MICB aa 23-309 (HA210907). |
| Positive control: | Recombinant Human MICB protein (HA210907). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-73] to Human MICB antibody (Capture) (HA723238) and Recombinant Human MICB protein (HA210907) as the standard. The reference range value is 39.1-5,000 pg/ml. |
| Uniprot #: | SwissProt: Q29980 Human |
| Alternative names: | MHC class I chain related protein B MHC class I chain related protein B2 MHC class I like molecule PERB11.2 IMX MHC class I mic B antigen MHC class I polypeptide related sequence B MHC class I polypeptide-related sequence B MIC B MIC-B MICB MICB_HUMAN PERB11.2 Stress inducible class I homolog |
Images
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Fig1:
Sandwich ELISA analysis of Human MICB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723238) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICB protein (HA210907) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723239, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native MICB in T-47D and PC-3 extract samples based on a 1,000 µg/ml extract load. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are T-47D extract 100% and PC-3 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 5,368 pg/ml in T-47D extract and undetectable in PC-3 extract. |
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Fig3:
Interpolated concentrations of native MICB in MCF-7 and PC-3 cell culture supernatant. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 100% and PC-3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 334.07 pg/ml in MCF-7 cell culture supernatant and undetectable in PC-3 cell culture supernatant. |
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Fig4:
Interpolated concentrations of spiked MICB in human cell culture media samples. The concentrations of MICB were interpolated from the MICB standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.