Human/Mouse VEGFC Recombinant Rabbit Monoclonal Antibody [PSH10-75]
cat.: HA723241
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH10-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VEGFC aa 103-227 (HA210869). |
| Positive control: | Recombinant Human VEGFC protein (HA210869). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-76] to Human/Mouse VEGFC antibody (Detector) (HA723242) and Recombinant Human VEGFC protein (HA210869) as the standard. The reference range value is 63-8,000 pg/ml. |
| Uniprot #: | SwissProt: P49767 Human | P97953 Mouse |
| Alternative names: | Flt 4L Flt4 ligand FLT4 ligand DHM Flt4-L Flt4L Vascular endothelial growth factor C Vascular endothelial growth factor related protein Vascular endothelial growth factor-related protein VEGF C VEGF-C Vegfc VEGFC_HUMAN VRP |
Images
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Fig1:
Sandwich ELISA analysis of Human VEGFC matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723241) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFC protein (HA210869) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723242, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native VEGF-C in human serum and mouse serum samples. The concentrations of VEGF-C were measured in duplicates, interpolated from the VEGF-C standard curve and corrected for sample dilution. Undiluted samples are human serum 100% and mouse serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-C concentration was determined to be 236.5 pg/ml in human serum and 115.4 pg/ml in mouse serum. |
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Fig3:
Interpolated concentrations of spiked VEGF-C in human cell culture media samples. The concentrations of VEGF-C were measured in duplicate, interpolated from the VEGF-C standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.