Human CSPG4 Recombinant Rabbit Monoclonal Antibody [PSH10-79]
cat.: HA723246
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH10-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CSPG4 aa 1356-2224 (HA211047). |
| Positive control: | Recombinant Human CSPG4 protein (HA211047). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-77] to Human CSPG4 antibody (Capture) (HA723244) or Rabbit monoclonal [PSH10-78] to Human CSPG4 antibody (Capture) (HA723245) and Recombinant Human CSPG4 protein (HA211047) as the standard. The reference range value is 156-20,000 pg/ml. |
| Uniprot #: | SwissProt: Q6UVK1 Human |
| Alternative names: | 4732461B14Rik AN2 AN2 proteoglycan Chondroitin sulfate proteoglycan 4 (melanoma-associated) Chondroitin sulfate proteoglycan 4 Chondroitin sulfate proteoglycan NG2 Cspg4 Cspg4 chondroitin sulfate proteoglycan 4 CSPG4_HUMAN HMW-MAA HSN tumor-specific antigen Kiaa4232 MCSP MCSPG MEL-CSPG Melanoma chondroitin sulfate proteoglycan Melanoma-associated chondroitin sulfate proteoglycan MELCSPG MSK16 NG2 |
Images
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Fig1:
Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723244) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723245) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig3:
Interpolated concentrations of native CSPG4 in A-375, SK-MEL-28, SK-BR-3 and MCF7 cell culture supernatant. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curve and corrected for sample dilution. Undiluted samples are A-375 cell culture supernatant 25%, SK-MEL-28 cell culture supernatant 100%, SK-BR-3 cell culture supernatant 100% and MCF7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSPG4 concentration was determined to be 164.9 ng/ml in A-375 cell culture supernatant, 17.2 ng/ml in SK-MEL-28 cell culture supernatant and undetectable in SK-BR-3 and MCF7 cell culture supernatant. |
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Fig4:
Interpolated concentrations of spiked CSPG4 in human cell culture media samples. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.