Cx40 / GJA5 Recombinant Rabbit Monoclonal Antibody [PSH10-82]
cat.: HA723249
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH10-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GJA5 aa 227-358. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, F9 cell lysate, COS-1 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate. |
| Subcellular location: | Cell membrane, Cell junction, gap junction. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P36382 Human | Q01231 Mouse | P28234 Rat |
| Alternative names: | Connexin 40 Connexin 40 / GJA5 Connexin-40 Cx40 CXA5_HUMAN Gap junction alpha 5 protein Gap junction alpha-5 protein Gap junction protein alpha 5 40kD (connexin 40) Gap junction protein alpha 5 Gja5 MGC11185 |
Images
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Fig1:
Western blot analysis of Cx40 / GJA5 on different lysates with Rabbit anti-Cx40 / GJA5 antibody (HA723249) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: F9 cell lysate (20 µg/Lane) Lane 5: COS-1 cell lysate (20 µg/Lane) Lane 6: Mouse lung tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723249) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Cx40 / GJA5 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA723249 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723249 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA723249 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA723249 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.