CD24 Recombinant Rabbit Monoclonal Antibody [PSH10-85]
cat.: HA723252
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, FC, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH10-85 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 8 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD24 aa 1-80. |
| Positive control: | MCF7, MCF7 cell lysate, HT-29 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Cell WB FC |
1:500 1:2,000-1:10,000 1:1,000 |
| Uniprot #: | SwissProt: P25063 Human |
| Alternative names: | CD 24 CD24 CD24 antigen (small cell lung carcinoma cluster 4 antigen) CD24 antigen CD24 molecule CD24_HUMAN CD24A FLJ22950 FLJ43543 GPI linked surface mucin Heat stable antigen HSA MGC75043 Nectadrin Signal transducer CD24 Small cell lung carcinoma cluster 4 antigen |
Images
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Fig1:
Immunocytochemistry analysis of MCF7 cells labeling CD24 with Rabbit anti-CD24 antibody (HA723252) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD24 antibody (HA723252) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig2:
Western blot analysis of CD24 on different lysates with Rabbit anti-CD24 antibody (HA723252) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 8 kDa Observed band size: 20-55 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723252) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of CD24 on different lysates with Rabbit anti-CD24 antibody (HA723252) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: HCT 116 cell lysate (negative) Lane 4: HT-29 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 8 kDa Observed band size: 25-40 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723252) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Flow cytometric analysis of MDA-MB-231 (left, negative) and MCF7 (right, positive) cells labeling CD24. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723252, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.