Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PSH10-87]
cat.: HA723254
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH10-87 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Gasdermin D aa 226-275. |
| Positive control: | THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate. |
| Subcellular location: | Cell membrane, Secreted, Mitochondrion membrane, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P57764 Human |
| Alternative names: | 1810036L03Rik DF 5L DF5L DFNA 5L DFNA5L FKSG 10 FKSG10 FLJ12150 Gasdermin Gasdermin D Gasdermin domain containing 1 Gasdermin domain containing protein 1 Gasdermin domain-containing protein 1 Gasdermin-D GasderminD GSDMD GSDMD_HUMAN GSDMDC 1 GSDMDC1 |
Images
|
Fig1:
Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA723254) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723254) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Gasdermin D (N terminal) was immunoprecipitated from 0.2 mg THP-1 treated with 80nM TPA for 16 hours then treated with 5μg/mL poly(dA:dT) for 3 hours cell lysate with HA723254 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723254 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 treated with 80nM TPA for 16 hours then treated with 5μg/mL poly(dA:dT) for 3 hours cell lysate (input) Lane 2: HA723254 IP in THP-1 treated with 80nM TPA for 16 hours then treated with 5μg/mL poly(dA:dT) for 3 hours cell lysate Lane 3: Rabbit IgG instead of HA723254 in THP-1 treated with 80nM TPA for 16 hours then treated with 5μg/mL poly(dA:dT) for 3 hours cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10econds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.