MICA + MICB Recombinant Rabbit Monoclonal Antibody [PSH10-88]
cat.: HA723255
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH10-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MICB aa 1-309. |
| Positive control: | K-562 cell lysate, HL-60 cell lysate, Jurkat cell lysate, HeLa cell lysate, HUVEC cell lysate, HeLa. |
| Subcellular location: | Cell membrane, Cytoplasm; Cell membrane. |
| Recommended Dilutions:
WB FC IP |
1:2,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q29980 Human | Q29983 Human |
| Alternative names: | HLA class I antigen FLJ36918 FLJ60820 MGC111087 MGC21250 MHC class I chain related gene A protein MHC class I chain related protein A MHC class I chain related protein A HLA B HLA C MHC class I polypeptide related sequence A MHC class I polypeptide-related sequence A MHC class I related protein MIC A MIC-A micA MICA_HUMAN OTTHUMP00000029088 OTTHUMP00000044528 OTTHUMP00000165170 OTTHUMP00000165172 PERB11.1 Stress inducible class I homolog MHC class I chain related protein B MHC class I chain related protein B2 MHC class I like molecule PERB11.2 IMX MHC class I mic B antigen MHC class I polypeptide related sequence B MHC class I polypeptide-related sequence B MIC B MIC-B MICB MICB_HUMAN PERB11.2 Stress inducible class I homolog |
Images
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Fig1:
Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: Jurkat cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: THP-1 cell lysate (negative) Lane 3: HUVEC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/2,000 dilution. Lane 1: Human MICA recombinant protein, 20ng/Lane Lane 2: Human MICB recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA + MICB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723255, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
MICA + MICB was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723255 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723255 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723255 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723255 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.