MAGI1 Recombinant Rabbit Monoclonal Antibody [PSH10-91]
cat.: HA723258
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH10-91
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 165 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MAGI1 aa 551-1,000.
Positive control: U-2 OS cell lysate, SW480 cell lysate, HepG2 cell lysate, HCT 116 cell lysate, HUVEC cell lysate, Mouse liver tissue lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Subcellular location: Cell junction, tight junction, Cell membrane.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q96QZ7 Human | Q6RHR9 Mouse | Q4L1J4 Rat
Alternative names: AIP 3 AIP-3 AIP3 Atrophin 1 interacting protein 3 Atrophin-1-interacting protein 3 BAI1 associated protein 1 BAI1-associated protein 1 BAIAP 1 BAIAP1 BAP 1 BAP-1 BAP1 Gukmi1 MAGI 1 MAGI-1 MAGI1 MAGI1_HUMAN MAGI1c Membrane associated guanylate kinase inverted 1 membrane associated guanylate kinase WW and PDZ domain containing 1 Membrane-associated guanylate kinase Membrane-associated guanylate kinase inverted 1 TNRC 19 TNRC19 Trinucleotide repeat containing gene 19 Trinucleotide repeat containing gene 19 protein Trinucleotide repeat-containing gene 19 protein WW and PDZ domain-containing protein 1 WW domain containing protein 3 WW domain-containing protein 3 WWP 3 WWP3
Images
HA723258_1.jpg Fig1: Western blot analysis of MAGI1 on different lysates with Rabbit anti-MAGI1 antibody (HA723258) at 1/2,000 dilution.

Lane 1: U-2 OS cell lysate (20 µg/Lane)
Lane 2: SW480 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (20 µg/Lane)
Lane 5: HUVEC cell lysate (20 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 8: Rat pancreas tissue lysate (40 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 165 kDa
Observed band size: 100-180 kDa

Exposure time: Lane 1-8: 2 minute 7 seconds; Lane 9-10: 25 seconds;ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723258) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.