| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH05-13 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Strep-tag II. |
| Recommended Dilutions:
WB |
1:2,000 |
| Alternative names: | anti-Strep-Tag II Strep-Tag II Strep-Tag II Strep-Tag IIantiboy |
|
Fig1:
Western blot analysis of Strep-Tag II on different lysates with Rabbit anti-Strep-Tag II antibody (HA723259) at 1/2,000 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate (20 µg/Lane) Lane 3: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate (10 µg/Lane) Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723259) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |