| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH10-94 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CPT1A aa 201-773 / 773. |
| Positive control: | Mouse liver tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Mitochondrion outer membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P97742 Mouse | P32198 Rat |
| Alternative names: | Carnitine O palmitoyltransferase 1 liver isoform Carnitine O palmitoyltransferase I Carnitine O palmitoyltransferase I liver isoform Carnitine O-palmitoyltransferase 1 Carnitine O-palmitoyltransferase I Carnitine palmitoyltransferase 1A (liver) Carnitine palmitoyltransferase 1A Carnitine palmitoyltransferase I Carnitine palmitoyltransferase I liver CPT 1 CPT I CPT1 CPT1 L CPT1-L Cpt1a CPT1A_HUMAN CPTI CPTI-L L CPT1 liver isoform |
|
Fig1:
Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (HA723261) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: Lane 1: 59 seconds; Lane 2: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723261) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |