AS160 Recombinant Rabbit Monoclonal Antibody [PSH10-95]
cat.: HA723262
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: PSH10-95
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 147 kDa
Isotype: IgG
Immunogen: Recombinant protein within human AS160 aa 1-500.
Positive control: HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, MCF7 cell lysate, Caco-2 cell lysate, human heart tissue, human skeletal muscle tissue, rat brain tissue, rat heart tissue, rat skeletal muscle tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IP
1:2,000-1:10,000
1:200-1:1,000
1-2μg/sample
Uniprot #: SwissProt: O60343 Human
Entrez Gene: 306117 Rat
Alternative names: Acrg embryonic lethality (mouse) minimal region ortholog Acrg embryonic lethality minimal region ortholog Acrg embryonic lethality mouse minimal region ortholog Akt substrate of 160 kDa AS 160 AS160 BUB2 CDC16 KIAA0603 NIDDM5 TBC (Tre 2 BUB2 CDC16) domain containing protein TBC Tre 2 BUB2 CDC16 domain containing protein TBC1 D4 TBC1 domain family member 4 Tbc1d4 TBCD4_HUMAN Tre-2
Images
HA723262_1.jpg Fig1: Western blot analysis of AS160 on different lysates with Rabbit anti-AS160 antibody (HA723262) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: MCF7 cell lysate (20 µg/Lane)
Lane 5: Caco-2 cell lysate (20 µg/Lane)

Predicted band size: 147 kDa
Observed band size: 160 kDa

Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723262) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723262_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-AS160 antibody (HA723262) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723262) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723262_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-AS160 antibody (HA723262) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723262) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723262_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-AS160 antibody (HA723262) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723262) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723262_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-AS160 antibody (HA723262) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723262) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723262_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-AS160 antibody (HA723262) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723262) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723262_7.jpg Fig7: AS160 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723262 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723262 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: HA723262 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA723262 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 9 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.