| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH10-97 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL-8 aa 1-99. |
| Positive control: | U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate, U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P10145 Human |
| Alternative names: | (Ala-IL-8)77 (Ser-IL-8)72 9E3 Beta thromboglobulin like protein C-X-C motif chemokine 8 CEF-4 chemokine, CXC motif, ligand 8 CXCL8 Emoctakin GCP-1 GCP/IL-8 protein I GCP/IL-8 protein II GCP/IL-8 protein III GCP/IL-8 protein IV GCP/IL-8 protein V GCP/IL-8 protein VI GCP1 Granulocyte chemotactic protein 1 IL-8 IL-8(1-77) IL-8(9-77) IL8 IL8/NAP1 form I IL8/NAP1 form II IL8/NAP1 form III IL8/NAP1 form IV IL8/NAP1 form V IL8/NAP1 form VI IL8_HUMAN Inteleukin 8 LECT LUCT Lymphocyte-derived neutrophil-activating factor LYNAP MDNCF MDNCF-b MDNCF-c MONAP Monocyte derived neutrophil activating peptide Monocyte derived neutrophil chemotactic factor Monocyte-derived neutrophil chemotactic factor Monocyte-derived neutrophil-activating peptide NAF NAP 1 NAP-1 NAP1 Neutrophil activating peptide 1 Neutrophil activating protein 1 Neutrophil-activating factor Neutrophil-activating protein 1 Protein 3 10C ...... |
|
Fig1:
Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (HA723264) at 1/2,000 dilution. Lane 1: U-937 cell lysate Lane 2: U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate Lane 3: U-87 MG cell lysate Lane 4: U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723264) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |